| 8564304 |
Toyama T, Teramoto H, Takeba G, Tsuji H: Cytokinin induces a rapid decrease in the levels of mRNAs for catalase, 3-hydroxy-3-methylglutaryl CoA reductase, lectin and other unidentified proteins in etiolated cotyledons of cucumber. Plant Cell Physiol. 1995 Oct;36(7):1349-59.
Large-scale differential hybridization was performed to examine rapid changes in gene expression caused by a phytohormone, cytokinin, in etiolated cotyledons of cucumber (Cucumis sativus L.). We isolated 86 cDNA clones for mRNAs whose levels decreased within 2 h of the start of treatment with N6-benzyladenine (BA). Partial nucleotide sequences showed that some of the cDNAs were homologous to those for catalase, 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) and a lectin. This is the first report that the levels of the mRNAs for those proteins are regulated by a cytokinin in darkness. Together with previous results [Teramoto et al. (1993) Physiol. Plant. 87: 584, (1994) Planta 193: 573, (1995) Planta 196: 387], the present study suggests that the cytokinin act to lower the levels of mRNAs transcribed from various genes in etiolated cotyledons. |
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