Protein Information

ID 1199
Name ribonuclease
Synonyms AGS 4; Ribonuclease; AGS4; JUNB; RNASEH2A; RNASEHI; RNHIA; RNHL…

Compound Information

ID 1714
Name gibberellic acid
CAS (1α,2β,4aα,4bβ,10β)-2,4a,7-trihydroxy-1-methyl-8-methylenegibb-3-ene-1,10-dicarboxylic acid 1,4a-lactone

Reference

PubMed Abstract RScore(About this table)
1002775 Gibson RA, Paleg LG: Purification of gibberellic acid-induced lysosomes from wheat aleurone cells. J Cell Sci. 1976 Nov;22(2):413-25.
Using isopycnic density gradient centrifugation, lysosomes were concentrated in a single region of a sucrose-Ficoll gradient (p = 1-10 g cm-3), well separated from most other cell organelles. Gibberellic acid-induced lysosomes were found to be rich in alpha-amylase and protease but not ribonuclease. The lysosomal band also contained a majority of the NADH2-cytochrome c reductase, a marker enzyme for endoplasmic reticulum, found in the gradient. Examination of electron micrographs revealed that a purified band of lyosomes contained at least 3 vesicle types, ranging in size from 0-1 to 0-5 mum. The significance of these findings to proposed mechanisms of action of gibberellic acid is discussed.
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