| 16272706 |
Uchiyama S, Ishiyama K, Hashimoto K, Yamaguchi M: Synergistic effect of beta-cryptoxanthin and zinc sulfate on the bone component in rat femoral tissues in vitro: the unique anabolic effect with zinc. Biol Pharm Bull. 2005 Nov;28(11):2142-5.
The effect of the combination of beta-cryptoxanthin and zinc sulfate (zinc) on bone components in the femoral-diaphyseal and -metaphyseal tissues of young rats in vitro was investigated. Bone tissues were cultured for 48 h in a serum-free Dulbecco's modified Eagle's medium containing either vehicle, beta-cryptoxanthin (10 (-9)-10 (-7) M) or zinc sulfate (10 (-6)-10 (-4) M). The presence of beta-cryptoxanthin (10 (-9) M) or zinc (10 (-6) M) did not have a significant effect on calcium content in the femoral-diaphyseal or -metaphyseal tissues. However, culture which combined beta-cryptoxanthin (10 (-9) M) and zinc (10 (-6) M) caused a significant increase in calcium content in the femoral-diaphyseal and -metaphyseal tissues. Such an effect was not observed by the combination of beta-cryptoxanthin (10 (-9) M) plus genistein (10 (-6) M) or menaquinone-7 (10 (-6) M), or zinc (10 (-6) M) plus genistein (10 (-6) M) or menaquinone-7 (10 (-6) M). Also, the combination of beta-cryptoxanthin (10 (-9) M) plus zinc (10 (-6) M) caused a remarkable increase in alkaline phosphatase activity and deoxyribonucleic acid (DNA) in the femoral-diaphyseal and -metaphyseal tissues, while their application alone did not have an effect on the enzyme activity or DNA content in the femoral tissues. The effect of the combination of beta-cryptoxanthin (10 (-9) M) plus zinc (10 (-6) M) in increasing calcium content, alkaline phosphatase activity, and DNA content in the femoral-diaphyseal and -metaphyseal tissues was completely prevented in the presence of cycloheximide (10 (-6) M), an inhibitor of protein synthesis, or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DBR), an inhibitor of transcriptional activity. This study demonstrates that the combination of beta-cryptoxanthin and zinc at a lower concentration has a synergistic effect on bone components in vitro. |
31(0,1,1,1) |