| 16730205 |
Choi YS, Lee KS, Yoon HJ, Kim I, Sohn HD, Jin BR: Bombus ignitus Cu,Zn superoxide dismutase (SOD1): cDNA cloning, gene structure, and up-regulation in response to paraquat, temperature stress, or lipopolysaccharide stimulation. Comp Biochem Physiol B Biochem Mol Biol. 2006 Jul;144(3):365-71. Epub 2006 Apr 7.
A Cu,Zn superoxide dismutase (SOD1) gene was cloned from the bumblebee, Bombus ignitus. The SOD1 gene of B. ignitus spans 1,317 bp and consists of three introns and four exons encoding 151 amino acid residues. The B. ignitus SOD1 (BiSOD1) possesses the typical metal-binding ligands of six histidines and one aspartic acid common to SOD1s. The deduced amino acid sequence of the BiSOD1 cDNA showed 82% identity to Apis mellifera SOD1 and 68-64% to SOD1 sequences from other insects. Northern blot analysis revealed the presence of BiSOD1 transcripts in all tissues examined. When paraquat (methyl viologen), a free radical-inducing agent, was injected into the body cavity of B. ignitus workers, BiSOD1 mRNA expression was up-regulated in the fat body. In addition, the expression levels of BiSOD1 mRNA in the fat body significantly increased when B. ignitus workers were exposed to low (4 degrees C) or high (37 degrees C) temperatures, or injected with lipopolysaccharide (LPS), which suggests that BiSOD1 possibly protects against oxidative stress caused by extreme temperatures and bacterial infection. |
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