| 19860477 |
Yang J, Rothery R, Sempombe J, Weiner JH, Kirk ML: Spectroscopic characterization of YedY: the role of sulfur coordination in a Mo (V) sulfite oxidase family enzyme form. J Am Chem Soc. 2009 Nov 4;131(43):15612-4.
Electronic paramagnetic resonance (EPR), electronic absorption, and magnetic circular dichroism spectroscopies have been performed on YedY, a SUOX fold protein with a Mo domain that is remarkably similar to that found in chicken sulfite oxidase, Arabidopsis thaliana plant sulfite oxidase, and the bacterial sulfite dehydrogenase from Starkeya novella. Low-energy dithiolene --> Mo and cysteine thiolate --> Mo charge-transfer bands have been assigned for the first time in a Mo (V) form of a SUOX fold protein, and the spectroscopic data have been used to interpret the results of bonding calculations. The analysis shows that second coordination sphere effects modulate dithiolene and cysteine sulfur covalency contributions to the Mo bonding scheme. In particular, a more acute O (oxo)-Mo-S (Cys)-C dihedral angle results in increased cysteine thiolate S --> Mo charge transfer and a large g (1) in the EPR spectrum. The spectrosocopic results, coupled with the available structural data, indicate that these second coordination sphere effects may play key roles in modulating the active-site redox potential, facilitating hole superexchange pathways for electron transfer regeneration, and affecting the type of reactions catalyzed by sulfite oxidase family enzymes. |
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