| 17117641 |
Zhu XS, Meng FP, Zhu L, He DH: [Flow injection biosensor based on the immobilized AChE] . Huan Jing Ke Xue. 2006 Sep;27(9):1829-34.
A biosensor based on the flow injection system was constructed with the immobilized AChE from Scomberomorus niphonius (Curier) as identification element and a pH electrode as transducer. When phosphate buffer was used as carrier liquid, a good reproducibility (RSD = 1.427% , n=10) of the biosensor response was obtained after the substrate was injected repetitively. After an incubation time of 20 min, the calibration graph to methyl-parathion is linear (r = 0.9986) when its concentration ranges from 4.29 x 10 (-10) mol x L (-1) to 4.29 x 10 (-8) mol x L (-1) , and the detection limit is 1.3 x 10 (-10) mol x L (-1). However, the sensitivity of this biosensor to methyl-parathion when using clean seawater as carrier liquid is not as good as that using phosphate buffer. But after preoxidation of methyl-parathion with NaClO as oxidant, the detection limit of the biosensor to methyl-parathion in seawater can be improve to 2.16 x 10 (-7) mol x L (-1). |
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