| 7136402 |
Lober M, Krantz S, Herrmann I: [Production and characterization of antisera against O,O-diethyl-O-(p-nitrophenyl)-phosphate (paraoxon)]. Acta Biol Med Ger. 1982;41(5):487-96.
Methods for the production of antibodies against Paraoxon in rabbits are described. The highest-titre antisera were produced with conjugates containing a succinyl spacer group with a degree of derivatisation between 8 and 12 haptens per albumin molecule. The antibody response was tested by immunoprecipitation in agar gel with a hapten-rabbit-albumin-complex and by a radioimmunoassay. The synthesis of 125I labelled tracers is reported. The most potent antisera bound 50% of the radioactive tracer after 1:10 (5) dilution and showed apparent affinity constants of 10 (6) to 10 (7) M-1 for Paraoxon. Compared to Paraoxon these antisera possessed cross reactivities with Parathion of 8.8 to 13.8%, with Methylparathion of 0.35 to 0.82%, and with Dimethoat of 0.0006 to 0.0012%. |
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