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Jyoti V, Narayan KD, Das SK: Gulbenkiania indiensis sp. nov., isolated from a sulfur spring. Int J Syst Evol Microbiol. 2009 Aug 7.
A bacterial strain, designated HT27T, was isolated from a sulfur spring sample collected from Athamallik, Orissa, India, and characterized using a polyphasic approach. This bacterium is Gram-negative, strictly aerobic, rod-shaped and motile with a single polar flagellum. Both oxidase and catalase activities are positive. Growth was observed at pH 5.0 to 11.0 and at temperature of 15 and 45 degrees C; higher growth yield was observed at pH 7.5-8.0 and 30-37 degrees C. The G+C content of genomic DNA is 63 mol%. The major cellular fatty acids are C16:1omega7c (44.24%), C16:0 (27.65%), C18:1omega7c (13.98%), C12:0 (2.60%) and C12:0 3-OH (2.22%). 16S rRNA gene sequence analysis indicated that the bacterium clustered with the genus Gulbenkiania and showed 99.0% similarity with Gulbenkiania mobilis (LMG 23770T). However, the strain HT27T showed 30% DNA-DNA reassociation with Gulbenkiania mobilis (LMG 23770T), the nearest phylogenetic relative. On the basis of the phenotypic, chemotaxonomic, 16S rRNA gene sequence analysis and DNA-DNA reassociation studies, it is proposed that strain HT27T be assigned as the type strain of a novel species of the genus Gulbenkiania, Gulbenkiania indiensis sp. nov. The GenBank accession number for the 16S rRNA gene sequence of Gulbenkiania indiensis is DQ415656. The type strain of Gulbenkiania indiensis sp. nov. is HT27T (=DSM 17901T = JCM 15969T). |
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