| 19500828 |
Wang XZ, Liu SS, Sun Y, Wu JY, Zhou YL, Zhang JH: Beta-cypermethrin impairs reproductive function in male mice by inducing oxidative stress. Theriogenology. 2009 Sep 15;72(5):599-611. Epub 2009 Jun 4.
Cypermethrin (CYP), an insecticide, has deleterious effects on male reproductive function. The objective was to identify whether the effects of beta-CYP on male reproductive organs were associated with oxidative stress. Three doses of beta-CYP (1, 10, and 20mg/kg) were administered to male mice for 35 d, with or without vitamin E (20mg/kg). The moderate (10mg/kg) and high (20mg/kg) doses of beta-CYP not only decreased body weight and the weight of the testes, epididymides, seminal vesicles, and prostate (P <0.05) but also reduced serum testosterone concentration and the expression of steroidogenic acute regulatory protein (P <0.05), in addition to damaging the seminiferous tubules and sperm development. Furthermore, moderate and high doses of beta-CYP administration decreased sperm number, sperm motility, and intact acrosome rate (P <0.05). Based on ultrastructural analyses, high doses of beta-CYP produced swelling and degeneration of mitochondria and the smooth endoplasmic reticulum of Leydig cells and caused the formation of concentric circles. These toxic effects of beta-CYP may be mediated by increasing oxidative stress, as the moderate and high doses of this compound increased malondialdehyde and nitric oxide in testes (P <0.05); reduced the activity of catalase, glutathione peroxidase (GSH-Px), and superoxide dismutase (P <0.05); and activated ERK1/2 (P <0.05). Vitamin E reversed the effects of beta-CYP on testosterone production and testis damage (P <0.05 vs. the high-dose group). Therefore, we inferred that beta-CYP damaged the structure of testes and decreased sperm output by inducing oxidative stress. |
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