19884787 |
Hamayun M, Khan SA, Khan AL, Rehman G, Sohn EY, Shah AA, Kim SK, Joo GJ, Lee IJ: Phoma herbarum as a new gibberellin-producing and plant growth-promoting fungus. J Microbiol Biotechnol. 2009 Oct;19(10):1244-9. Endophytic fungi are known for the production of valuable metabolites, but information on the gibberellin production capacity of this group is limited. We isolated 9 endophytic fungi from the roots of salt-stressed soybean plants and screened them on waito-c rice, in order to identify plant growth promoting fungal strains. The fungal isolate TK- 2-4 gave maximum plant length (20.35 cm) promotion in comparison with wild-type Gibberella fujikuroi (19.5 cm). In a separate experiment, bioassay of TK-2-4 promoted plant length and biomass of soybean cultivar Taegwangkong. The TK-2-4 culture filtrate was analyzed for the presence of gibberellins, and it was found that all physiologically active gibberellins, especially GA (4) and GA (7), were present in higher amounts (GA (1), 0.11 ng/ml; GA (3), 2.91 ng/ml; GA (4), 3.21 ng/ml; and GA (7), 1.4 ng/ml) in conjunction with physiologically inactive GA (9) (0.05 ng/ml), GA (12) (0.23 ng/ ml), GA (15) (0.42 ng/ml), GA (19) (0.53 ng/ml), and GA (20) (0.06 ng/ml). The fungal isolate TK-2-4 was later identified as a new strain of Phoma herbarum, through the phylogenetic analysis of 28S rDNA sequence. |
8(0,0,1,3) |