| 213050 |
Kolk AH, van Kuyk L, Boettcher B: Isolation of human lactate dehydrogenase isoenzyme X by affinity chromatography. Biochem J. 1978 Sep 1;173(3):767-71.
Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the other isoenzymes. The purified human isoenzyme LDH-X had a specific activity of 146 units/mg of protein. |
32(0,1,1,2) |