| 2735254 |
Uchijima Y, Yoshida K, Saito H: A further study of human seminal plasma lactate dehydrogenase-C4 (LDH-C4): kinetic properties of LDH-C4. Hinyokika Kiyo. 1989 Mar;35(3):457-63.
Lactate dehydrogenase-C4 (LDH-C4) from human seminal plasma was purified by 40-60% (NH4) 2SO4 precipitation, Oxamate-Sepharose 4B affinity column chromatography and AMP affinity column chromatography. Enzyme kinetic studies of human seminal plasma LDH-C4 were performed with purified LDH -C4. The human LDH-C4 exhibits a much higher affinity for pyruvate and alpha-ketovalerate than lactate. Apparent Km values for pyruvate, alpha-ketovalerate and lactate were 7.7 X 10 (-5) M, 1.3 X 10 (-3) M and 6.7 X 10 (-3) M, respectively. The human seminal plasma LDH-C4 was severely inhibited by increasing concentration of substrate, especially lactate. These kinetic properties were compared with those of previous reports of human spermatozoa. |
32(0,1,1,2) |