| 9428654 |
Wong C, Rodriguez-Paez L, Nogueda B, Perez A, Baeza I: Selective inhibition of the sperm-specific lactate dehydrogenase isozyme-C4 by N-isopropyl oxamate. Biochim Biophys Acta. 1997 Nov 14;1343(1):16-22.
In the present study, we demonstrated that the attachment of the nonpolar isopropylic carbon chain in the nitrogen of oxamate, converted this competitive inhibitor of LDH isozymes into a powerful selective inhibitor of mouse LDH-C4. The comparative study of the inhibitory effect of oxamate and N-isopropyl oxamate on mouse LDH isozymes pointed out that the isopropylic carbon chain conferred upon N-isopropyl oxamate a high affinity for LDH-C4 and a marked decrease in the affinity for the other isozymes since oxamate showed more inhibitory effect on LDH-1 (Ki = 0.06 mM) and LDH-5 (Ki = 0.08 mM), and less inhibitory effect on LDH-C4 (Ki = 0.25 mM). On the other hand, N-isopropyl oxamate showed the highest inhibitory effect on LDH-C4 (Ki = 0.014 mM) and poor inhibitory effect on LDH-1 (Ki = 0.4 mM) and LDH-5 (Ki = 0.8 mM). Apparently, the enzymatic inactivation proceeded through a reversible binding of N-isopropyl oxamate, facilitated by nonpolar interactions with a hydrophobic region present only in the active site of mouse LDH-C4, resulting in a selective inhibition of this isozyme in comparison with the other LDH isozymes. N-isopropyl oxamate was also a powerful competitive inhibitor of LDH-C4 (Ki = 0.015 mM) compared with oxamate (Ki = 0.35 mM), using alpha-ketoisocaproate as a substrate. |
31(0,1,1,1) |