| 12549198 |
Guo W, He X, Tie C, Zhang B: [Expression of alpha-acetolactate decarboxylase gene from Bacillus subtilis in Brewer's yeast]. Wei Sheng Wu Xue Bao. 2001 Feb;41(1):105-8.
Yeast YIp-type expression recombinant plasmid (pCMA2-1) was constructed. The expression of alpha-acetolactate decarboxylase gene from Bacillus subtilis was controled by CUP1 promoter and its own terminator. The recombinant plasmid pCMA2-1 was introduced into the brewer's yeast PJ3-5. Transformants were selected using copper resistance as selected marker. The results of activity assay showed that PJ3-5 didn't produce alpha-acetolactate decarboxylase (ALDC), where as the activity of alpha-ALDC in transformants were induced by copper sulfate. The laboratory scale fermentation test confirmed that the total diacetyl concentration was reduced effectively by alpha-ALDC in transformant. |
7(0,0,1,2) |