| 17720863 |
Li HY, Park CK, Jung SJ, Choi SY, Lee SJ, Park K, Kim JS, Oh SB: Eugenol inhibits K+ currents in trigeminal ganglion neurons. . J Dent Res. 2007 Sep;86(9):898-902.
Eugenol, a natural capsaicin congener, is widely used in dentistry. Eugenol inhibits voltage-activated Na (+) and Ca (2+) channels in a transient receptor potential vanilloid 1 (TRPV1)-independent manner. We hypothesized that eugenol also inhibits voltage-gated K (+) currents, and investigated this in rat trigeminal ganglion neurons and in a heterologous system using whole-cell patch clamping. Eugenol inhibited voltage-gated K (+) currents, and the inhibitory effects of eugenol were observed in both capsaicin-sensitive and capsaicin-insensitive neurons. Pre-treatment with capsazepine, a well-known antagonist of TRPV1, failed to block the inhibitory effects of eugenol on K (+) currents, suggesting no involvement of TRPV1. Eugenol inhibited human Kv1.5 currents stably expressed in Ltk (-) cells, where TRPV1 is not endogenously expressed. We conclude that eugenol inhibits voltage-gated K (+) currents in a TRPV1-independent manner. The inhibition of voltage-gated K (+) currents is likely to contribute to the irritable action of eugenol. Abbreviations: human Kv1.5 channel, hKv1.5; transient receptor potential vanilloid 1, TRPV1. |
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