| 16137013 |
Wan WQ, Liu XY, Deng B: [Mechanisms of dibutyl phthalate to purge leukemic cells] . Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Feb;29(1):79-80.
OBJECTIVE: To determine the mechanism of dibutyl phthalate (DBP) to purge leukemic cells by inducing apoptosis. METHODS: The effects of DBP on the intracellular free Ca2+ concentration ([Ca2+] i) of leukemic cells were measured by Fura-2AM method. The effects of DBP on the protein expression of c-myc and bcl-2 genes of leukemic cells were measured by immunohistochemical assay. RESULTS: DBP elicited an intracellular Ca2+ redistribution and a potent extracellular calcium influx in leukemic cells. It also down-regulated the protein expression of c-myc and bcl-2 genes. CONCLUSION: DBP can purge leukemic cells in vitro by increasing [Ca2+] i in cells to initiate apoptosis and down-regulate bcl-2 and c-myc proto-gene expression to promote the cell apoptosis. |
3(0,0,0,3) |