| 9409001 |
Toida T, Shima M, Azumaya S, Maruyama T, Toyoda H, Imanari T, Linhardt RJ: Detection of glycosaminoglycans as a copper (II) complex in high-performance liquid chromatography. J Chromatogr A. 1997 Nov 7;787(1-2):266-70.
Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper (II) complex in copper sulfate solution at low pH. Detection of the copper (II)-heparin complex is sensitive, permitting the analysis of as little as 10 (-7) g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N-acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases. |
1(0,0,0,1) |