| 15861238 |
Wang JJ, Cheng WX, Ding W, Zhao ZM: The effect of the insecticide dichlorvos on esterase activity extracted from the psocids, Liposcelis bostrychophila and L. entomophila. J Insect Sci. 2004;4:23. Epub 2004 Jul 14.
The inhibition kinetics of dichlorvos on carboxylesterase and acetylcholinesterase (AChE) activity extracted from Liposcelis bostrychophila and L. entomophila (Psocoptera: Liposcelididae) were compared. The results showed that L. entomophila had significantly greater specific activity of carboxylesterase than L. bostrychophila (0.045 versus 0.012 micromoles/mg/min). Moreover, the carboxylexterase of L. entomophila showed higher affinity (i.e. lower Km value) to the substrate 1-naphthyl acetate than L. bostrychophila (0.29 versus 0.67 mM). The specific activity and affinity of AChE of the two species were not significantly different. The carboxylesterase of L. bostrychophila was more sensitive to the insecticide dichlorvos than that of L. entomophila. The I50s values of dichlorvos to carboxylesterase for L. bostrychophila and L. entomophila were 1.43 and 3.28 microM, respectively, and to AChE were 324 and 612 nM, respectively. Inhibition kinetics revealed that AChE from L. bostrychophila was 5.8-fold more sensitive to inhibition than AChE from L. entomophila. |
88(1,1,2,3) |