| 17639959 |
Jia KZ, Li XH, He J, Gu LF, Ma JP, Li SP: [Isolation of a monocrotophos-degrading bacterial strain and characterization of enzymatic degradation]. Huan Jing Ke Xue. 2007 Apr;28(4):908-12.
A monocrotophos [dimethyl (E)-1-2-methylcarbamoylvinylphosphate or MCP] -degrading strain named as M-1 was isolated from sludge collected from the wastewater treatment pool of a pesticide factory and identified as Paracoccus sp. according to its morphology and biochemical properties and 16S rDNA sequence analysis. Using MCP as a sole carbon source, M-1 was able to degrade MCP (100 mg x L (-1)) by 92.47% in 24 h. The key enzyme (s) involved in the initial biodegradation of monocrotophos in M-1 was shown to be constitutively expressed cytosolic proteins and showed the greatest activity at pH 8.0 and 25 degrees C, with its Michaelis-Mentn's constant (K (m)) and maximum degradation rate (V (max)) of 0.29 micromol x mL (-1) and 682.12 micromol (min x mg)(-1) respectively. This degrading enzyme (s) was sensitive to high temperature, but kept high activity under alkaline conditions. |
7(0,0,1,2) |