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de la Fuente T, Martin-Fontecha M, Sallander J, Benhamu B, Campillo M, Medina RA, Pellissier LP, Claeysen S, Dumuis A, Pardo L, Lopez-Rodriguez ML: Benzimidazole derivatives as new serotonin 5-HT6 receptor antagonists. J Med Chem. 2010 Feb 11;53(3):1357-69.
Molecular mechanisms of receptor inactivation.. On the basis of our previously described pharmacophore model for serotonin 5-HT (6) receptor (5-HT (6) R) antagonists, we have designed, synthesized, and pharmacologically characterized a series of benzimidazole derivatives 1-20 that represent a new family of potent antagonists at the human 5-HT (6) R. Site-directed mutagenesis and a beta (2)-adrenoceptor-based homology model of the 5-HT (6) R were used to predict the mode of binding of antagonist SB-258585 and the new synthesized ligands. Substitution of W6.48, F6.52, or N6.55 by Ala fully impedes compound 4 to block 5-HT-induced activation. Thus, we propose that D3.32 in TM 3 anchors the protonated piperazine ring, the benzimidazole ring expands parallel to EL 2 to hydrogen bond N6.55 in TM 6, and the aromatic ring is placed between TMs 3 and 5 in CH (2)-containing compounds and between TMs 3 and 6 in CO-containing compounds. This combined experimental and computational study has permitted to propose the molecular mechanisms by which the new benzimidazole derivatives act as 5-HT (6) R antagonists. |
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