| 11601686 |
Sanchez M, Gella FJ, Profilis C, Ceriotti F, Cuso E, Fuentes-Arderiu X, Hallermayer K, Horder M, Panteghini M, Schumann G, Canalias F: Certification of the mass concentration of creatine kinase isoenzyme 2 (CK-MB) in the reference material BCR 608. Clin Chem Lab Med. 2001 Sep;39(9):858-65.
We describe the certification of a mass concentration value in the already prepared creatine kinase-2 reference material (BCR 608). Creatine kinase-2 was purified from human heart. The purified enzyme was diluted in order to measure its protein concentration by the Doetsch method. A protein concentration value of 124.30+/-13.17 mg/l was assigned to the stock solution of purified creatine kinase-2. This stock solution was diluted in 25 mmol/l piperazine-N,N'-bis [2-ethanesulfonic acid] (PIPES) pH 7.2, containing 2 mmol/l ADP, 5 mmol/l 2-mercaptoethanol, 154 mmol/l sodium chloride and 50 g/l human albumin to obtain a stable liquid standard of known creatine kinase-2 mass concentration (80.36 microg/l). This standard was then used to recalculate the creatine kinase-2 mass concentration measured in the BCR 608 material by immunoassay. The mass concentration of creatine kinase-2 in samples of reconstituted BCR 608 was certified to be 93.30+/-9.65 microg/l. |
6(0,0,1,1) |