Protein Information

Name albumin
Synonyms ALB; Albumin; PRO0883; PRO0903; PRO1341; PRO1708; PRO2044; PRO2619…

Compound Information

Name copper sulfate
CAS sulfuric acid copper(2+) salt (1:1)

Reference List

PubMed Abstract RScore(About this table)
20180041 Naseri Z, Mohri M, Aslani MR, Alavi Tabatabaee AA: Effects of Short-Term Over-supplementation of Copper in Milk on Hematology, Serum Proteins, Weight Gain, and Health in Dairy Calves. Biol Trace Elem Res. 2010 Feb 24.

From 14 days of age, in the test 1 group copper as copper sulfate (Merck Co, Germany) was added to each meal of milk at a rate of 10 mg/kg of milk for 14 days and in test 2 group copper as copper sulfate was added to each meal of milk at a rate of 20 mg/kg of milk for 14 days.
Plane tubes were used for harvesting of serum and the amounts of total serum protein, albumin, iron, and copper were measured.
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17575443 Kawada E, Moridaira K, Sato H, Ukita T, Itoh K, Tatsumi T, Kogure T, Tamura J, Sakamoto H: [Effects of copper supplementation on lipid profiles in elderly patients with copper deficiency]. Nippon Ronen Igakkai Zasshi. 2007 May;44(3):375-9.

METHODS: Nine long-term bed-ridden, patients (5 men and 4 women, mean age 83.3+/-8.7 years old) with severe copper deficiency, who had a serum copper of 15 microg/dL or less (normal range 70-140 microg/dL), had their diets supplemented with copper sulfate (3 mg/day) over 12 weeks in addition to their diet of only one kind of enteral food with a low concentration of copper.
Copper, ceruloplasmin, total cholesterol (TC), triacylglycerides (TG), HDL-cholesterol (HDL-C), c-reactive protein (CRP), creatinine (Cr), zinc (Zn) and albumin (Alb) in the serum were measured before, 4 weeks and 12 weeks after copper supplementation.
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9545555 Adhikari S, Joshi R, Gopinathan C: Bilirubin as an anti precipitant against copper mediated denaturation of bovine serum albumin: formation of copper-bilirubin complex. Biochim Biophys Acta. 1998 Mar 12;1380(1):109-14.

The rate of formation of this Cu-BR complex when BR extracts copper from copper-albumin complex as obtained in our experiment is 34.981 mol-1 s-1.
Addition of excess copper sulfate to the solution of this complex causes a greenish black precipitate which can be re dissolved in HCl but insoluble in 99% ethanol.
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18346470 Amirtharaj GJ, Natarajan SK, Mukhopadhya A, Zachariah UG, Hegde SK, Kurian G, Balasubramanian KA, Ramachandran A: Fatty acids influence binding of cobalt to serum albumin in patients with fatty liver. Biochim Biophys Acta. 2008 May;1782(5):349-54. Epub 2008 Feb 29.


Oxidative stress can modify albumin and affect ligand binding.
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8485631 Berman RS, Martin W: Arterial endothelial barrier dysfunction: actions of homocysteine and the hypoxanthine-xanthine oxidase free radical generating system. Br J Pharmacol. 1993 Apr;108(4):920-6.


Endothelial barrier function was assessed by use of an in vitro model in which transfer of trypan blue-labelled albumin was measured across monolayers of bovine aortic endothelial cells grown on polycarbonate membranes. 2.
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6234304 Anderson WL, Tomasi TB: Suppression of lymphocyte proliferation by copper-albumin chelates. . J Biol Chem. 1984 Jun 25;259(12):7602-6.

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19873279 Anson ML: SOME FACTORS WHICH INFLUENCE THE OXIDATION OF SULFHYDRYL GROUPS. J Gen Physiol. 1942 Jan 20;25(3):355-367.

Cyanide inhibits the oxidation of the SH groups of cysteine and denatured egg albumin by the uric acid reagent. 2.
At pH 4.8 cysteine is oxidized by the uric acid reagent and by ferricyanide in the presence but not in the absence of added copper sulfate. 3.
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8906595 Viens L, Athias A, Lizard G, Simard G, Gueldry S, Braschi S, Gambert P, Lallemant C, Lagrost L: Effect of lipid transfer activity and lipolysis on low density lipoprotein (LDL) oxidizability: evidence for lipolysis-generated non-esterified fatty acids as inhibitors of LDL oxidation. J Lipid Res. 1996 Oct;37(10):2179-92.

Subsequently, modified LDL were oxidized with copper sulfate and the extent of LDL oxidation was evaluated.
Non-esterified fatty acids (NEFA) arose as putative candidates in reducing oxidation susceptibility of LDL: NEFA-containing LDL were less oxidizable; the enrichment of LDL with either oleic acid or linoleic acid reduced significantly their oxidability; the oxidation susceptibility of either in vitro modified LDL or LDL isolated from normal or analbuminemic patients significantly increased after reduction of their NEFA content with fatty acid-poor albumin.
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3673601 Dimitrova D, Radonova N, Ivancheva E, Russanov E: The hemolytic effect of copper complexes with different ligands. . Acta Physiol Pharmacol Bulg. 1987;13(2):48-53.


The hemolytic effect of Cu (II) complexes was abolished by strong complexons of Cu (II) and by physiological concentrations of albumin and histidine.
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16327069 Arabi M, Alaeddini MA: Metal-ion-mediated oxidative stress in the gill homogenate of rainbow trout (Oncorhynchus mykiss): antioxidant potential of manganese, selenium, and albumin. Biol Trace Elem Res. 2005 Winter;108(1-3):155-68.

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10750174 Tokarnia CH, Dobereiner J, Peixoto PV, Moraes SS: Outbreak of copper poisoning in cattle fed poultry litter. Vet Hum Toxicol. 2000 Apr;42(2):92-5.

Copper poisoning was diagnosed, most probably caused by feeding litter from poultry that had been fed a ration treated with copper sulfate to avoid aspergillosis.
Changes in the kidneys included nephrosis and the presence of bile and precipitates, and cylinders of albumin and of hemoglobin in the uriniferous tubules.
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9296445 Proudfoot JM, Puddey IB, Beilin LJ, Stocker R, Croft KD: Unexpected dose response of copper concentration on lipoprotein oxidation in serum: discovery of a unique peroxidase-like activity of urate/albumin in the presence of high copper concentrations. Free Radic Biol Med. 1997;23(5):699-705.

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