| 17520089 |
Kuciel R, Mazurkiewicz A, Dudzik P: Kinetic intermediates of unfolding of dimeric prostatic phosphatase. Acta Biochim Pol. 2007;54(2):371-7. Epub 2007 May 23.
Kinetics of guanidine hydrochloride (GdnHCl)-induced unfolding of human prostatic acid phosphatase (hPAP), a homodimer of 50 kDa subunit molecular mass was investigated with enzyme activity measurements, capacity for binding an external hydrophobic probe, 1-anilinonaphtalene-8-sulfonate (ANS), accessibility of thiols to reaction with 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) and 2-(4'-maleimidylanilino) naphthalene-6-sulfonate (MIANS) and ability to bind Congo red dye. |
31(0,1,1,1) |
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