| 19959275 |
Gildenhuys S, Dobreva M, Kinsley N, Sayed Y, Burke J, Pelly S, Gordon GP, Sayed M, Sewell T, Dirr HW: Arginine 15 stabilizes an S (N) Ar reaction transition state and the binding of anionic ligands at the active site of human glutathione transferase A1-1. Biophys Chem. 2010 Feb;146(2-3):118-25. Epub 2009 Nov 18.
Arg15 in human GSTA1-1 was replaced with a leucine and crystallographic, spectroscopic, thermodynamic and molecular docking methods were used to investigate the contribution made by Arg15 towards (i) the binding of glutathione (GSH) to the G-site, (ii) the pK (a) of the thiol group of GSH, (iii) the stabilization of an analog of the anionic transition state of the S (N) Ar reaction between 1-chloro-2,4-dinitrobenzene (CDNB) and GSH, and, (iv) the binding of the anionic non-substrate ligand 8-anilino-1-naphthalene sulphonate (ANS) to the H-site. |
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