Protein Information

Name prothrombin
Synonyms Coagulation factor II; Coagulation factor II variant; F2; F2 protein; F2 protein precursor; Factor II; PT; Prothrombin…

Compound Information

Name sodium thiocyanate
CAS sodium thiocyanate

Reference List

PubMed Abstract RScore(About this table)
3531198 Speijer H, Govers-Riemslag JW, Zwaal RF, Rosing J: Prothrombin activation by an activator from the venom of Oxyuranus scutellatus (Taipan snake). J Biol Chem. 1986 Oct 5;261(28):13258-67.

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3748998 Jenny R, Church W, Odegaard B, Litwiller R, Mann K: Purification of six human vitamin K-dependent proteins in a single chromatographic step using immunoaffinity columns. Prep Biochem. 1986;16(3):227-45.

Purified monoclonal antibodies and a purified burro polyclonal anti-prothrombin immunoglobulin were individually coupled to Sepharose and used in a tandem series of columns to purify each of the vitamin K-dependent proteins from eluates of barium citrate precipitates of plasma.
The proteins were eluted from the columns by sodium thiocyanate and retained functional activity following dialysis.
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7654686 Zondlo J, Fisher KE, Lin Z, Ducote KR, Eisenstein E: Monomer-heptamer equilibrium of the Escherichia coli chaperonin GroES. Biochemistry. 1995 Aug 22;34(33):10334-9.


In an effort to clarify the role of GroES in chaperonin-facilitated protein folding, a plasmid-encoding expression system for GroES incorporating a histidine-tagged, thrombin-cleavable, N-terminal sequence was constructed.
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