Protein Information

Name C18
Synonyms B1; D1; BBS 9; BBS9; BBS9 GENE; Bardet Biedl syndrome 9 protein; C18; PTH responsive osteosarcoma B1 protein…

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
8958151 Antosiewicz J, Damiani E, Jassem W, Wozniak M, Orena M, Greci L: Influence of structure on the antioxidant activity of indolinic nitroxide radicals. Free Radic Biol Med. 1997;22(1-2):249-55.

Three distinct indolinic nitroxides were synthesized and compared with vitamin E and Trolox (a hydrophilic analogue of vitamin E), where the only difference between the nitroxides was the length of the hydrocarbon chain in the 2-position of indole, namely 2 (C2), 10 (C10), and 18 (C18) carbons.
Mitochondrial rotenone-sensitive NADH oxidase and succinate oxidase responded differently to BuOOH-induced radical chemistry, and the compounds under study also protected the activity of the two enzymes but to different extents.
2(0,0,0,2) Details
11529183 Draper WM, Dhoot JS, Perera SK: Determination of rotenoids and piperonyl butoxide in water, sediments and piscicide formulations. J Environ Monit. 1999 Dec;1(6):519-24.

Rotenone is a naturally occurring insecticide and piscicide (fish poison) found in many leguminous plants.
Compounds were separated on a C18 reversed phase column with an acetonitrile-0.025 M phosphoric acid mobile phase and detected by UV absorbance or fluorescence (PBO only).
2(0,0,0,2) Details
12058901 Martel AC, Zeggane S: Determination of acaricides in honey by high-performance liquid chromatography with photodiode array detection. J Chromatogr A. 2002 Apr 19;954(1-2):173-80.

For thymol, one clean up on a solid-phase extraction C18 (500 mg, 6 ml) column was performed; for rotenone, a liquid extraction with dichloromethane was realised.
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12541972 Zeng XN, Coll J, Zhang SX, Liu XQ, Camps F: [Modification of the analytical method for rotenoids in plants] . Se Pu. 2002 Mar;20(2):144-7.

However, the HPLC method available for this purpose was developed particularly for the detection and determination of rotenone, so it appears to be unsuitable for the analysis of other rotenoids such as deguelin, elliptone and their analogues.
The extraction of rotenoids from plants is conveniently carried out by CHCl3-MeOH (9:1, V/V) and the purification can be accomplished by filtration of the crude residue through a C18 reversed-phase cartridge.
1(0,0,0,1) Details
3730409 Veerkamp JH, Zevenbergen JL: Effect of dietary fat on total and peroxisomal fatty acid oxidation in rat tissues. Biochim Biophys Acta. 1986 Aug 14;878(1):102-9.

Oxidation of [1-14C]- and [16-14C] palmitate was assayed in the absence as well as in the presence of antimycin plus rotenone in homogenates of liver, heart and skeletal muscle of four groups of rats fed diets containing 40 energy% fat of different fatty acid composition.
Three groups were given fat blends rich in C16, C18 saturated (cocoa butter), cis-monounsaturated (low-linoleic-acid olive oil) or trans fatty acids (partially hydrogenated soybean oil), respectively.
1(0,0,0,1) Details
12568369 Holm A, Molander P, Lundanes E, Greibrokk T: Determination of rotenone in river water utilizing packed capillary column switching liquid chromatography with UV and time-of-flight mass spectrometric detection. J Chromatogr A. 2003 Jan 3;983(1-2):43-50.

Sample volumes of up to 1 ml are loaded onto a 23 x 0.25 mm, 5 microm Kromasil C18 packed capillary precolumn using a noneluting solvent composition of water-acetonitrile (99:1, v/v) at flow-rates up to 100 microl/min prior to solute backflushing onto a 200 x 0.32 mm, 3.5 microm Kromasil C18 packed capillary analytical column using a mobile phase of water-acetonitrile (30:70, v/v) at a flow-rate of 5 microl/min.
1(0,0,0,1) Details