| 19286013 |
Granja RH, Nino AM, Zucchetti RA, Nino RE, Patel R, Salerno AG: Determination of streptomycin residues in honey by liquid chromatography-tandem mass spectrometry. Anal Chim Acta. 2009 Apr 1;637(1-2):64-7. Epub 2009 Jan 10.
Streptomycin is an aminoglycoside antibiotic used in apiculture to protect bees against a variety of brood diseases. Brazilian authorities have included it in the National Regulatory Monitoring Program for honey production. A simple and reliable method using liquid chromatography-tandem mass spectrometry has been developed and validated for the determination of streptomycin in honey. The chromatography separation was performed on a Gemini 5 microm C18 (50 mm x 2 mm) column using 5mM heptafluorbutiric acid/acetonitrile (85:15) as the mobile phase at a flow rate of 0.2 mLmin (-1). The detection of the analyte was achieved by positive ionization electrospray in multiple reaction-monitoring modes. Two characteristic transitions were monitored for streptomycin. Some analytical parameters were validated according to the guidelines laid down by European Commission Decision 2002/657/EC: decision limit, detection capability, recovery, precision and ruggedness. The recoveries of streptomycin from honey fortified at 2.5, 10, 15 and 20 microgkg (-1) levels are around 100%. The decision limit and detection capability of streptomycin was 3 microgkg (-1) and 4.7 microgkg (-1) respectively. |
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