| ID | 63 |
|---|---|
| Name | phosphatidylinositol specific |
| Synonyms | PLC beta 3; Phosphoinositide phospholipase C; PLCB 3; PLCB3; Phosphatidylinositol specific; Phospholipase C beta 3; Phosphatidylinositol specifics; Phospholipase C beta 3s |
| ID | 730 |
|---|---|
| Name | OCH |
| CAS | 2,3,4,4,5,5,6,6-octachloro-2-cyclohexen-1-one |
| PubMed | Abstract | RScore(About this table) |
|---|---|---|
| 18191462 | Schmolke M, Fabian J, Lehr M: High-performance liquid chromatographic assay with ultraviolet spectrometric detection for the evaluation of inhibitors of phosphatidylinositol-specific phospholipase C. Anal Biochem. 2008 Apr 15;375(2):291-8. Epub 2007 Dec 25. A nonradioactive spectrometric assay for the evaluation of inhibitors of phosphatidylinositol-specific phospholipase C (PI-PLC) is described. l-alpha-Phosphatidylinositol from bovine liver was used as substrate in the presence of the micelle-forming detergent PI-PLC isolated from Bacillus cereus and crude cytosol fractions from porcine brain were used as enzyme sources. PI-PLC activity was determined by measuring the release of 1-stearoyl-2-arachidonoyl-sn- with reversed-phase HPLC and UV detection at 200 nm. PI-PLC from B. cereus was not inhibited by the putative PI-PLC inhibitors U-73122 and ET-18-OCH (3) at 100 microM, whereas the isobenzofuranone derivative 5 blocked the enzyme with an IC (50) of 75 microM. PI-PLC activity present in porcine brain cytosol was decreased by all three test compounds at 100 microM to approximately 30 to 50%. |
2(0,0,0,2) |