Protein Information

ID 1206
Name Lactoferrin
Synonyms GIG12; Growth inhibiting protein 12; HLF 2; HLF2; LF; LTF; Lactoferrin; Lactotransferrin…

Compound Information

ID 1388
Name sodium fluoride
CAS sodium fluoride (NaF)

Reference

PubMed Abstract RScore(About this table)
15203033 Schlatterer B, Baeker R, Schlatterer K: Improved purification of beta-lactoglobulin from acid whey by means of ceramic hydroxyapatite chromatography with sodium fluoride as a displacer. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Aug 5;807(2):223-8.
The successful separation of beta-lactoglobulin from other bovine whey proteins was performed by ceramic hydroxyapatite chromatography with a fluoride ion gradient in phosphate buffer as displacement agent. The method was applied to acid whey originating from milk of healthy as well as of mastitic cows. beta-Lactoglobulin was completely eluted in one peak at a fluoride concentration of about 0.6 mol/l. The purity of beta-lactoglobulin in this fraction was at least 96% if whey from healthy milk was processed. Co-eluted contaminants are traces of immunoglobulin G, serum albumin and lactoferrin. In case of mastitic whey the proportion of beta-lactoglobulin is diminished as the amounts of immunglobulin G, serum albumin and lactoferrin are increased within this fraction. Size exclusion chromatography on Superdex 75 pg effectively removed contaminants resulting in a purity for beta-lactoglobulin from normal whey of approximately 99%. The yield of beta-lactoglobulin from physiological whey was 50-55% referring to the fraction highly enriched with beta-lactoglobulin by hydroxyapatite chromatography. In case of mastitic milk the higher amounts of contaminants were also removed successfully by size exclusion chromatography.
2(0,0,0,2)