| 12504354 |
Chen J, Shan KR, Long YG, Wang YN, Nordberg A, Guan ZZ: Selective decreases of nicotinic acetylcholine receptors in PC12 cells exposed to fluoride. Toxicology. 2003 Feb 1;183(1-3):235-42.
In an attempt to elucidate the mechanism by which excessive fluoride damages the central nervous system, the effects of exposure of PC12 cells to different concentrations of fluoride for 48 h on nicotinic acetylcholine receptors (nAChRs) were characterized here. Significant reductions in the number of binding sites for both [3H] epibatidine and [125I] alpha-bungarotoxin, as well as a significant decrease in the B (max) value for the high-affinity of epibatidine binding site were observed in PC12 cells subjected to high levels of fluoride. On the protein level, the alpha 3 and alpha 7 subunits of nAChRs were also significantly decreased in the cells exposed to high concentrations of fluoride. In contrast, such exposure had no significant effect on the level of the beta 2 subunit. These findings suggest that selective decreases in the number of nAChRs may play an important role in the mechanism (s) by which fluoride causes dysfunction of the central nervous system. |
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