| 17241545 |
Deng J, Chen GY, Ji JJ, Tan H, Wang S, Hu FX, Chai LH, Liu SH, Liu WD: [Apoptosis and gene FasL expression induced by carbon disulfide in rat steroli cells]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Dec;24(12):730-3.
OBJECTIVE: To study apoptosis and gene FasL expression induced by carbon disulfide in steroli cells of male rats. METHODS: Steroli cells were exposed to different concentrations of CS (2) (0, 0.36, 0.72, 1.44 micromol/ml) for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively. RESULTS: Steroli cell survival rate decreased as the concentration of CS (2) increased. The survival rate (73.34% +/- 1.39%) was significantly lower than the control group (99.98% +/- 5.48%) when the concentration of CS (2) > or = 1.44 micromol/ml (P < 0.05). Apoptosis rate increased as the CS (2) concentration increased. Apoptosis rate (7.93% +/- 0.43%) was significantly higher when the concentration of CS (2) > or = 1.44 micromol/ml (P < 0.05). Expression level of the FasL significantly increased as the concentrations of CS (2) (P < 0.05). CONCLUSION: CS (2) is cytotoxic to steroli cells. It could cause apoptosis of steroli cells. |
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