| 18331041 |
Kim HM, Jeong BH, Hyon JY, An MJ, Seo MS, Hong JH, Lee KJ, Kim CH, Joo T, Hong SC, Cho BR: Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue. J Am Chem Soc. 2008 Apr 2;130(13):4246-7. Epub 2008 Mar 11.
We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl) amino] naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 mum in live tissues using two-photon microscopy. |
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