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Earley FG, Patel SD, Ragan I, Attardi G: Photolabelling of a mitochondrially encoded subunit of NADH dehydrogenase with [3H] dihydrorotenone. FEBS Lett. 1987 Jul 13;219(1):108-12. Mitochondrial NADH dehydrogenase from bovine heart was photolabelled with the inhibitor [3H] dihydrorotenone. A constituent of the hydrophobic domain of the enzyme of Mr 33,000 was the major site of labelling. The identity of this protein with the mitochondrially encoded ND-1 gene product was established by immunoblotting and immunoprecipitation with an antiserum raised to the expected C-terminal sequence of the human ND-1 gene product. |
2(0,0,0,2) |