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Gurtubay JI, Martinez J, Gutierrez-Arranz A, Goni FM: Assay of mitochondrial membrane-bound enzyme activities in the presence of triton X-100. Rev Esp Fisiol. 1979 Dec;35(4):395-400.
Mitochondrial ATPase and cytochrome c oxidase activities are not severely affected by Triton X-100 concentrations between 0.1 and 2.0% (w/v). The former is solubilized by the effect of the detergent, while the latter is not. Succinate: cytochrome c reductase and rotenone-sensitive NADH: cytochrome c reductase activities are destroyed even a low detergent concentrations. Succinate:coenzyme Q oxidoreductase is affected by the surfactant in a more complex way, so that selective solubilization of some subunit (s) could be involved. |
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