| 2894857 |
Sambasivarao D, Kramer R, Rao NM, Sitaramam V: ATP hydrolysis induces variable porosity to mannitol in the mitochondrial inner membrane. Biochim Biophys Acta. 1988 Mar 30;933(1):200-11.
Osmotic titration of ATPase activity in rat liver mitochondria was consistent with enhanced porosity of the mitochondrial inner membrane to mannitol due to ATP hydrolysis even when endogenous respiration was inhibited by rotenone. The occluded ATPase activity, which exhibits osmotic activation with an optimum near isotonicity, depends both on the ATPase activity per se and on the activity of the ADP/ATP carrier. Purified ADP/ATP carrier incorporated into small, unilamellar liposomes was critically shown to exhibit dependence of its activity on the osmotic pressure differences across the membrane, with maximal activity corresponding to isotonicity, regardless of the actual internal tonicity. |
32(0,1,1,2) |