| 19486898 |
Jung SH, Lee ST, Chu K, Park JE, Lee SU, Han TR, Kim M: Cell proliferation and synaptogenesis in the cerebellum after focal cerebral ischemia. Brain Res. 2009 Aug 11;1284:180-90. Epub 2009 May 30.
Cerebral ischemia induces cortical function reorganization and neocortical neurogenesis in the cerebral hemisphere. However, whether the cerebellum undergoes corresponding dynamic change after cerebral injury has not been determined. We investigated the characteristics of cell proliferation and synaptogenesis in the cerebellum after focal cerebral ischemia. After induction of focal cerebral ischemia in rats, bromodeoxyuridine (BrdU, 100 mg/kg, intraperitoneally, daily) was administered for 5 days. Brain sections were analyzed using immunohistochemistry at days 7, 14, and 28. BrdU+ cells and Iba-1+ cells were counted, and cerebellar synaptophysin immunoreactivity was quantitatively analyzed. Rats with cerebral ischemia showed increased numbers of BrdU+ cells in the cerebellum at day 28 compared to sham-operated rats. Neither neuronal markers nor GABAergic markers were co-labeled with BrdU+ cells, suggesting that none of the proliferating cells contributed to neurogenesis. Instead, the number of Iba-1+ cells in the cerebellum was increased, which suggests that microglia are activated in the cerebellum after cerebral ischemia. The optical density of synaptophysin in the cerebellum of rats with cerebral ischemia was significantly increased in the molecular and granular layers at days 7 and 14. Cerebellar synaptophysin expression was significantly correlated with cerebellar cell proliferation. This study shows that the cerebellum undergoes specific and dynamic changes at the cellular level after focal cerebral ischemia, including cell proliferation and synaptogenesis. |
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