| 7612992 |
Ueki T, Sawada Y, Fukagawa Y, Oki T: Arylsulfatase from Streptomyces griseorubiginosus S980-14. Biosci Biotechnol Biochem. 1995 Jun;59(6):1062-8.
A new arylsulfatase designated Es-1, which desulfated etoposide 4'-sulfate and p-nitrophenyl sulfate, was isolated from Streptomyces griseorubiginosus S980-14 and purified to protein homogeneity by ammonium sulfate fractionation, ion exchange column chromatography, and chromatofocusing. The enzyme was active in monomeric form with an approximate molecular weight of 45,000, had a pI value of 4.95, and required calcium for full activity. At an optimum reaction pH of 8.5, iodoacetate, mercurous chloride, and EDTA severely inhibited the activity of Es-1 arylsulfatase. |
83(1,1,1,3) |