| 9754925 |
Tapia JC, Aguilar LF, Sotomayor CP, Aguayo LG: Ethanol affects the function of a neurotransmitter receptor protein without altering the membrane lipid phase. J Neurophysiol. 2007 Dec;98(6):3666-76. Epub 2007 Oct 3.
Using patch-clamp and fluorescence techniques we found that ethanol (10-200 mM) potentiated strychnine-sensitive glycine receptors without having detectable effects on lipid order parameters in mouse spinal cord neurons. Hepthanol (0.01-1 mM), in contrast, did not affect the glycine current, but it altered the core and surface of spinal neuron membranes as detected by changes in 1,6-diphenyl-1,3,5-hexatriene (DPH) and Laurdan fluorescence parameters. These findings support the idea that ethanol affects these membrane proteins without changing lipid fluidity. |
6(0,0,1,1) |