| 10938323 |
Chery N, De Koninck Y: GABA (B) receptors are the first target of released GABA at lamina I inhibitory synapses in the adult rat spinal cord. J Physiol. 2003 Nov 1;552(Pt 3):975-91. Epub 2003 Aug 22.
We have previously provided functional evidence that glycine and GABA are contained in the same synaptic vesicles and coreleased at the same synapses in lamina I of the rat spinal dorsal horn. However, whereas both glycine receptors (GlyRs) and GABA (A) receptors (GABA (A) Rs) are expressed on the postsynaptic target, under certain conditions inhibitory events appeared to be mediated by GlyRs only. We therefore wanted to test whether GABA (B) receptors could be activated in conditions where GABA released was insufficient to activate GABA (A) Rs. Focal stimulation in the vicinity of visually identified lamina I neurons elicited monosynaptic IPSCs in the presence of ionotropic glutamate receptor antagonists. Pairs of stimuli were given at different interstimulus intervals (ISI), ranging from 25 ms to 1 s to study the depression of the second of evoked IPSCs (paired pulse depression; PPD). Maximal PPD of IPSCs was 60 +/- 14% (SE) (of the conditioning pulse amplitude), at ISI between 150 and 200 ms. PPD was observed with IPSCs evoked at stimulus intensities where they had no GABA (A) R component. PPD of small evoked IPSCs was not affected by the GABA (A) R antagonist bicuculline but significantly attenuated by 10-30 microM CGP52432, a specific GABA (B) receptor antagonist. These data indicate that, under conditions where GABA released is insufficient to affect postsynaptic GABA (A) Rs at lamina I inhibitory synapses, significant activation of presynaptic GABA (B) receptors can occur. |
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