| Name | cytochrome P450 (protein family or complex) |
|---|---|
| Synonyms | cytochrome P450; cytochrome P 450; CYP450; CYP 450 |
| Name | brassinolide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 15016564 | Kim TW, Chang SC, Lee JS, Hwang B, Takatsuto S, Yokota T, Kim SK: Cytochrome P450-catalyzed brassinosteroid pathway activation through synthesis of castasterone and brassinolide in Phaseolus vulgaris. Phytochemistry. 2004 Mar;65(6):679-89. |
83(1,1,1,3) | Details |
| 16024588 | Kim TW, Hwang JY, Kim YS, Joo SH, Chang SC, Lee JS, Takatsuto S, Kim SK: Arabidopsis CYP85A2, a cytochrome P450, mediates the Baeyer-Villiger oxidation of castasterone to brassinolide in brassinosteroid biosynthesis. Plant Cell. 2005 Aug;17(8):2397-412. Epub 2005 Jul 15. |
82(1,1,1,2) | Details |
| 9675902 | Mathur J, Molnar G, Fujioka S, Takatsuto S, Sakurai A, Yokota T, Adam G, Voigt B, Nagy F, Maas C, Schell J, Koncz C, Szekeres M: Transcription of the Arabidopsis CPD gene, encoding a steroidogenic cytochrome P450, is negatively controlled by brassinosteroids. Plant J. 1998 Jun;14(5):593-602. The Arabidopsis CPD gene encodes a cytochrome P450 steroid side-chain hydroxylase (CYP90) that plays an essential role in the biosynthesis of the plant hormone brassinolide. |
7(0,0,1,2) | Details |
| 11389832 | Kang JG, Yun J, Kim DH, Chung KS, Fujioka S, Kim JI, Dae HW, Yoshida S, Takatsuto S, Song PS, Park CM: Light and brassinosteroid signals are integrated via a dark-induced small G protein in etiolated seedling growth. Cell. 2001 Jun 1;105(5):625-36. Here, we demonstrate that a dark-induced small G protein, pea Pra2, regulates a variant cytochrome P450 that catalyzes C-2 hydroxylation in brassinosteroid biosynthesis. Transgenic plants with reduced Pra2 exhibit a dark-specific dwarfism, which is completely rescued by exogenous brassinolide. |
3(0,0,0,3) | Details |
| 17073784 | Bishop G, Nomura T, Yokota T, Montoya T, Castle J, Harrison K, Kushiro T, Kamiya Y, Yamaguchi S, Bancos S, Szatmari AM, Szekeres M: Dwarfism and cytochrome P450-mediated C-6 oxidation of plant steroid hormones. Biochem Soc Trans. 2006 Dec;34(Pt 6):1199-201. Some of the most recent observations include the fact that certain CYP85 CYPs catalyse the synthesis of the most bioactive BR, BL (brassinolide). |
2(0,0,0,2) | Details |
| 8612270 | Szekeres M, Nemeth K, Koncz-Kalman Z, Mathur J, Kauschmann A, Altmann T, Redei GP, Nagy F, Schell J, Koncz C: Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell. 1996 Apr 19;85(2):171-82. The cpd mutation localized by T-DNA tagging on Arabidopsis chromosome 5-14.3 inhibits cell elongation controlled by the ecdysone-like brassinosteroid hormone brassinolide. |
2(0,0,0,2) | Details |
| 15705958 | Tanabe S, Ashikari M, Fujioka S, Takatsuto S, Yoshida S, Yano M, Yoshimura A, Kitano H, Matsuoka M, Fujisawa Y, Kato H, Iwasaki Y: A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. Plant Cell. 2005 Mar;17(3):776-90. Epub 2005 Feb 10. The dwarf phenotype of d11 mutants was restored by the application of the brassinolide (BL). |
2(0,0,0,2) | Details |
| 14615594 | Hong Z, Ueguchi-Tanaka M, Umemura K, Uozu S, Fujioka S, Takatsuto S, Yoshida S, Ashikari M, Kitano H, Matsuoka M: A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. Plant Cell. 2003 Dec;15(12):2900-10. Epub 2003 Nov 13. The dwarf phenotype of d2 was rescued by exogenous brassinolide treatment. |
2(0,0,0,2) | Details |
| 12226529 | Bancos S, Nomura T, Sato T, Molnar G, Bishop GJ, Koncz C, Yokota T, Nagy F, Szekeres M: Regulation of transcript levels of the Arabidopsis cytochrome p450 genes involved in brassinosteroid biosynthesis. Plant Physiol. 2002 Sep;130(1):504-13. Semiquantitative reverse transcriptase-polymerase chain reaction analyses show that transcript levels of CYP85 and CYP90 genes are down-regulated by brassinolide, the end product of the BR biosynthesis pathway. |
2(0,0,0,2) | Details |
| 16872648 | Ohnishi T, Nomura T, Watanabe B, Ohta D, Yokota T, Miyagawa H, Sakata K, Mizutani M: Tomato cytochrome P450 CYP734A7 functions in brassinosteroid catabolism. . Phytochemistry. 2006 Sep;67(17):1895-906. Epub 2006 Jul 25. By measuring the Type I substrate-binding spectra using recombinant CYP734A7, the dissociation constants for castasterone, brassinolide, and 6-deoxocastasterone were determined to be 6.7, 12, and 12 microM, respectively. |
1(0,0,0,1) | Details |
| 18175930 | Sekimata K, Ohnishi T, Mizutani M, Todoroki Y, Han SY, Uzawa J, Fujioka S, Yoneyama K, Takeuchi Y, Takatsuto S, Sakata K, Yoshida S, Asami T: Brz220 interacts with DWF4, a cytochrome P450 monooxygenase in brassinosteroid biosynthesis, and exerts biological activity. Biosci Biotechnol Biochem. 2008 Jan;72(1):7-12. Epub 2008 Jan 7. This step is catalyzed by DWF4, an Arabidopsis cytochrome P450 identified as a steroid 22-hydroxylase. |
1(0,0,0,1) | Details |
| 15689343 | Nakamura M, Satoh T, Tanaka S, Mochizuki N, Yokota T, Nagatani A: Activation of the cytochrome P450 gene, CYP72C1, reduces the levels of active brassinosteroids in vivo. J Exp Bot. 2005 Mar;56(413):833-40. Epub 2005 Feb 2. Furthermore, the hypocotyl phenotype was restored by the addition of brassinolide to the culture medium, suggesting that brassinosteroids had been affected in this mutant. |
1(0,0,0,1) | Details |
| 9490746 | Choe S, Dilkes BP, Fujioka S, Takatsuto S, Sakurai A, Feldmann KA: The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22alpha-hydroxylation steps in brassinosteroid biosynthesis. Plant Cell. 1998 Feb;10(2):231-43. Dwarfism could be rescued by the application of brassinolide, suggesting that DWF4 plays a role in brassinosteroid (BR) biosynthesis. |
1(0,0,0,1) | Details |
| 17693126 | Bishop GJ: Refining the plant steroid hormone biosynthesis pathway. Trends Plant Sci. 2007 Sep;12(9):377-80. Epub 2007 Aug 10. Many of the biochemical conversions in plant steroid hormone biosynthesis are catalysed by cytochrome P450 enzymes (CYPs or P450s). A recent paper by Toshiyuki Ohnishi et al. (2006) indicates the role of CYP90C1 and CYP90D1 in the synthesis of the most bioactive plant steroid hormone, brassinolide. |
1(0,0,0,1) | Details |
| 9990098 | Bishop GJ, Nomura T, Yokota T, Harrison K, Noguchi T, Fujioka S, Takatsuto S, Jones JD, Kamiya Y: The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis. Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1761-6. The tomato Dwarf gene (D), which was predicted to encode a cytochrome P450 enzyme (P450) with homology to other P450s involved in BR biosynthesis, was cloned previously. Here, we show that DWARF catalyses the C-6 oxidation of 6-deoxocastasterone (6-deoxoCS) to castasterone (CS), the immediate precursor of brassinolide. |
1(0,0,0,1) | Details |
| 11319239 | Asami T, Mizutani M, Fujioka S, Goda H, Min YK, Shimada Y, Nakano T, Takatsuto S, Matsuyama T, Nagata N, Sakata K, Yoshida S: Selective interaction of triazole derivatives with DWF4, a cytochrome P450 monooxygenase of the brassinosteroid biosynthetic pathway, correlates with brassinosteroid deficiency in planta. J Biol Chem. 2001 Jul 13;276(28):25687-91. Epub 2001 Apr 23. The levels of castasterone and brassinolide in brassinazole-treated plant cells were less than 6% of the levels in untreated cells. DWF4, which is an Arabidopsis thaliana cytochrome P450 isolated as a putative steroid 22-hydroxylase, was expressed in Escherichia coli, and the binding affinity of brassinazole and its derivatives to the recombinant DWF4 were analyzed. |
1(0,0,0,1) | Details |
| 15064374 | Saito S, Hirai N, Matsumoto C, Ohigashi H, Ohta D, Sakata K, Mizutani M: Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid. Plant Physiol. 2004 Apr;134(4):1439-49. Epub 2004 Apr 2. The hydroxylation at the 8'-position of ABA is known as the key step of ABA catabolism, and this reaction is catalyzed by ABA 8'-hydroxylase, a cytochrome P450. |
1(0,0,0,1) | Details |
| 11319033 | Pien S, Wyrzykowska J, Fleming AJ: Novel marker genes for early leaf development indicate spatial regulation of carbohydrate metabolism within the apical meristem. Plant J. 2001 Mar;25(6):663-74. The other two genes identified encoded a Phantastica-like myb transcription factor (associated with the acquisition of leaf dorsiventrality) and CYP85 (a cytochrome P450, which plays a pivotal role in brassinolide metabolism). The other two genes identified encoded a Phantastica-like myb transcription factor (associated with the acquisition of leaf dorsiventrality) and CYP85 (a cytochrome P450, which plays a pivotal role in brassinolide metabolism). |
1(0,0,0,1) | Details |
| 15710611 | Nomura T, Kushiro T, Yokota T, Kamiya Y, Bishop GJ, Yamaguchi S: The last reaction producing brassinolide is catalyzed by cytochrome P-450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis. J Biol Chem. 2005 May 6;280(18):17873-9. Epub 2005 Feb 14. Previous work has shown that the formation of castasterone from 6-deoxocastasterone is catalyzed by members of the CYP85A family of cytochrome P-450 monooxygenases. |
1(0,0,0,1) | Details |