Protein Information

Name RNase P (protein family or complex)
Synonyms RNase P; Ribonuclease P

Compound Information

Name blasticidin-S
CAS

Reference List

PubMed Abstract RScore(About this table)
12688530 Kalavrizioti D, Vourekas A, Tekos A, Tsagla A, Stathopoulos C, Drainas D: Kinetics of inhibition of ribonuclease P activity by peptidyltransferase inhibitors. Mol Biol Rep. 2003 Mar;30(1):9-14.

A cell-free system derived from Dictyostelium discoideum has been used to study the kinetics of inhibition of RNase P by puromycin, amicetin and blasticidin S.
113(1,2,2,3) Details
16337609 Hung CF, Cheng TL, Wu RH, Teng CF, Chang WT: A novel bidirectional expression system for simultaneous expression of both the protein-coding genes and short hairpin RNAs in mammalian cells. Biochem Biophys Res Commun. 2006 Jan 27;339(4):1035-42. Epub 2005 Dec 1.

In mammals, the conserved poly (ADP-ribose) polymerase 2 (PARP-2)/RNase P bidirectional control promoter simultaneously expresses both the PARP-2 protein and RNase P RNA by RNA polymerase II- and III-dependent mechanisms, respectively.
In addition, to extend its utility for the establishment of inherited stable clones, we have also reconstructed this bidirectional expression system with the blasticidin S deaminase gene, an effective dominant drug resistance selectable marker, and examined both the selection and inhibition efficiencies in drug resistance and gene expression.
2(0,0,0,2) Details
11092557 Stathopoulos C, Tsagla A, Tekos A, Drainas D: Effect of peptidyltransferase inhibitors on ribonuclease P activity from Dictyostelium discoideum. Mol Biol Rep. 2000 Jun;27(2):107-11.

Effect of antibiotics on RNase P..
Among the inhibitors tested puromycin, amicetin and blasticidin S revealed a dose-dependent inhibition of tRNA maturation.
1(0,0,0,1) Details