Protein Information

Name transferase
Synonyms 4' phosphopantetheinyl transferase; 4' phosphopantetheinyl transferase; AASD PPT; AASDHPPT; AASDPPT; Alpha aminoadipic semialdehyde dehydrogenase phosphopantetheinyl transferase; Aminoadipate semialdehyde dehydrogenase phosphopantetheinyl transferase; CGI 80…

Compound Information

Name blasticidin-S
CAS

Reference List

PubMed Abstract RScore(About this table)
7707371 Rodriguez-Fonseca C, Amils R, Garrett RA: Fine structure of the peptidyl transferase centre on 23 S-like rRNAs deduced from chemical probing of antibiotic-ribosome complexes. J Mol Biol. 1995 Mar 24;247(2):224-35.

Ribosomal binding sites were investigated for the diverse group of antibiotics: anisomycin, anthelmycin, blasticidin S, bruceantin, carbomycin, chloramphenicol, griseoviridin, narciclasine, T2 toxin, tylosin and virginiamycin M1 all of which are considered to inhibit the peptidyl transferase reaction by different mechanisms.
86(1,1,1,6) Details
239742 Yukioka M, Hatayama T, Morisawa S: Affinity labeling of the ribonucleic acid component adjacent to the peptidyl recognition center of peptidyl transferase in Escherichia coli ribosomes. Biochim Biophys Acta. 1975 May 1;390(2):192-208.

The alkylation was strongly enhanced by poly (U), and was dependent on the presence of both 50- and 30-S subunits; Chloramphenicol inhibited the reaction, wheras blasticidin S stimulated it.
3(0,0,0,3) Details
12860128 Hansen JL, Moore PB, Steitz TA: Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit. J Mol Biol. 2003 Jul 25;330(5):1061-75.

Structures of anisomycin, chloramphenicol, sparsomycin, blasticidin S, and virginiamycin M bound to the large ribosomal subunit of Haloarcula marismortui have been determined at 3.0A resolution.
2(0,0,0,2) Details
849441 Sikorski MM, Cerna J, Rychlik I, Legocki AB: Peptidyl transferase activity in wheat germ ribosomes. Biochim Biophys Acta. 1977 Mar 2;475(1):123-30.

The fragment reaction is inhibited by sparsomycin, blasticidin S, gougerotin and to a lesser degree by amicetin and tetracycline.
1(0,0,0,1) Details
9154836 Iordanov MS, Pribnow D, Magun JL, Dinh TH, Pearson JA, Chen SL, Magun BE: Ribotoxic stress response: activation of the stress-activated protein kinase JNK1 by inhibitors of the peptidyl transferase reaction and by sequence-specific RNA damage to the alpha-sarcin/ricin loop in the 28S rRNA. Mol Cell Biol. 1997 Jun;17(6):3373-81.

1(0,0,0,1) Details
371683 Gonzalez A, Vazquez D, Jimenez A: Inhibition of translation in bacterial and eukaryotic systems by the antibiotic anthelmycin (hikizimycin). Biochim Biophys Acta. 1979 Feb 27;561(2):403-9.

The drug is structurally similar to certain other 4-aminohexosyl cytosine antibiotics including blasticidin S, gougerotin, amicetin and bamicetin although unlike these compounds anthelmycin lacks an aminoacyl moiety.
It is proposed that anthelmycin inhibits the ribosomal peptidyl transferase centre by associating with a site that overlaps the (related) ribosomal receptor site (s) for the other four inhibitors.
1(0,0,0,1) Details