Protein Information

Name ubiquitin
Synonyms HMG20; RPS27A; UBA80; UBCEP 1; UBCEP1; UBA52; UBCEP 2; UBCEP2…

Compound Information

Name TCA
CAS 2,2,2-trichloroacetic acid

Reference List

PubMed Abstract RScore(About this table)
17241446 Shlevin L, Regev-Rudzki N, Karniely S, Pines O: Location-specific depletion of a dual-localized protein. Traffic. 2007 Feb;8(2):169-76.


The depletion of the protein occurs post-translationally via degradation by the ubiquitin-proteasome system, which operates only in the cytosol and the nucleus.
3(0,0,0,3) Details
17101781 Pan Y, Mansfield KD, Bertozzi CC, Rudenko V, Chan DA, Giaccia AJ, Simon MC: Multiple factors affecting cellular redox status and energy metabolism modulate hypoxia-inducible factor prolyl hydroxylase activity in vivo and in vitro. Mol Cell Biol. 2007 Feb;27(3):912-25. Epub 2006 Nov 13.


Prolyl hydroxylation of hypoxible-inducible factor alpha (HIF-alpha) proteins is essential for their recognition by pVHL containing ubiquitin ligase complexes and subsequent degradation in oxygen (O (2))-replete cells.
1(0,0,0,1) Details
15917633 Carlson E, Bays N, David L, Skach WR: Reticulocyte lysate as a model system to study endoplasmic reticulum membrane protein degradation. Methods Mol Biol. 2005;301:185-205.


These substrates can be rapidly isolated in native ER membranes which, when incubated in RRL lacking exogenous hemin, are degraded in an ATP-dependent manner by the ubiquitin-proteasome pathway.
1(0,0,0,1) Details