Protein Information

Name acetyl CoA synthetase
Synonyms ACAS 2; ACS; MYH7B; Acetyl Coenzyme A synthetase 2; ACAS2; ACSA; ACSS 2; ACSS2…

Compound Information

Name TCA
CAS 2,2,2-trichloroacetic acid

Reference List

PubMed Abstract RScore(About this table)
19861411 Jensen PR, Peitersen T, Karlsson M, In 't Zandt R, Gisselsson A, Hansson G, Meier S, Lerche MH: Tissue-specific short chain fatty acid metabolism and slow metabolic recovery after ischemia from hyperpolarized NMR in vivo. J Biol Chem. 2009 Dec 25;284(52):36077-82. Epub 2009 Oct 27.


This is achieved by assaying acetyl-CoA synthetase and acetyl-carnitine transferase catalyzed transformations in vivo.
2(0,0,0,2) Details
16310273 Li M, Ho PY, Yao S, Shimizu K: Effect of lpdA gene knockout on the metabolism in Escherichia coli based on enzyme activities, intracellular metabolite concentrations and metabolic flux analysis by 13C-labeling experiments. J Biotechnol. 2006 Mar 23;122(2):254-66. Epub 2005 Nov 23.


Based on the investigation of enzyme activities and intracellular metabolite concentrations, acetyl-CoA was considered to be formed by the combined reactions through pyruvate oxidase (PoxB), acetyl-CoA synthetase (Acs) and acetate kinase (Ack)-phosphoacetyltransferase (Pta) in the lpdA mutant.
1(0,0,0,1) Details
16551598 Wen L, Liu G, Zhang ZJ, Tao J, Wan CX, Zhu YG: [Preliminary proteomics analysis of the total proteins of HL Type cytoplasmic male sterility rice anther]. Yi Chuan. 2006 Mar;28(3):311-6.


Among those proteins, there were Putative nucleic acid binding protein, glucose-1-phosphate adenylyltransferase (ADP-glucose pyrophosphorylase, AGPase) (EC: 2.7.7.27) large chain, UDP-glucuronic acid decarboxylase, putative calcium-binding protein annexin, putative acetyl-CoA synthetase and putative lipoamide dehydrogenase etc.
3(0,0,0,3) Details
15063611 Phue JN, Shiloach J: Transcription levels of key metabolic genes are the cause for different glucose utilization pathways in E. coli B (BL21) and E. coli K (JM109). J Biotechnol. 2004 Apr 8;109(1-2):21-30.


In addition, there is a difference between the two strains in the transcription of the acetyl-CoA synthetase (acs), phosphotransacetylase-acetate kinase (pta-ackA) pathway, and pyruvate oxidase (poxB), pathway.
1(0,0,0,1) Details
15806547 Phue JN, Noronha SB, Hattacharyya R, Wolfe AJ, Shiloach J: Glucose metabolism at high density growth of E. coli B and E. coli K: differences in metabolic pathways are responsible for efficient glucose utilization in E. coli B as determined by microarrays and Northern blot analyses. Biotechnol Bioeng. 2005 Jun 30;90(7):805-20.


In a series of previous reports it was established by implementing metabolic flux, NMR/MS, and Northern blot analysis that the glyoxylate shunt, the TCA cycle, and acetate uptake by acetyl-CoA synthetase are more active in Escherichia coli BL21 than in Escherichia coli JM109.
1(0,0,0,1) Details
16209543 Wang Y, Wu SL, Hancock WS, Trala R, Kessler M, Taylor AH, Patel PS, Aon JC: Proteomic profiling of Escherichia coli proteins under high cell density fed-batch cultivation with overexpression of phosphogluconolactonase. Biotechnol Prog. 2005 Sep-Oct;21(5):1401-11.


During the time course of samples collected during the rapid growth achieved under batch and fed-batch conditions, both the control and recombinant E. coli strains showed up-regulation of proteins participating in the tricarboxylic acid (TCA) cycle, particularly acetyl-CoA synthetase (AcCoAS), malate dehydrogenase (MDH), and succinyl-CoA synthetase (SuccCoAS).
1(0,0,0,1) Details