Protein Information

Name phosphofructokinase
Synonyms PFK 1; Phosphofructokinase 1; Phosphohexokinase; PFK A; PFK1; PFKM; PFKM protein; Phosphofructo 1 kinase isozyme A…

Compound Information

Name TCA
CAS 2,2,2-trichloroacetic acid

Reference List

PubMed Abstract RScore(About this table)
20064709 Chen XC, Song H, Fang T, Cao JM, Ren HJ, Bai JX, Xiong J, Ouyang PK, Ying HJ: Enhanced cyclic adenosine monophosphate production by Arthrobacter A302 through rational redistribution of metabolic flux. Bioresour Technol. 2010 May;101(9):3159-63. Epub 2010 Jan 12.


Meanwhile, the specific activities of glyceraldehyde 3-phosphate dehydrogenase, phosphofructokinase and pyruvate kinase were decreased by 66%, 61%, and 46%, respectively.
1(0,0,0,1) Details
19788729 Furch T, Preusse M, Tomasch J, Zech H, Wagner-Dobler I, Rabus R, Wittmann C: Metabolic fluxes in the central carbon metabolism of Dinoroseobacter shibae and Phaeobacter gallaeciensis, two members of the marine Roseobacter clade. BMC Microbiol. 2009 Sep 29;9:209.


Enzyme assays confirmed this flux pattern and link the lack of glycolytic flux to the absence of phosphofructokinase activity.
1(0,0,0,1) Details
19603213 Wu L, Li Z, Ye Q: Enhanced D-ribose biosynthesis in batch culture of a transketolase-deficient Bacillus subtilis strain by citrate. J Ind Microbiol Biotechnol. 2009 Oct;36(10):1289-96. Epub 2009 Jul 15.


Addition of citrate reduced the activities of pyruvate kinase and phosphofructokinase, while it increased those of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase.
1(0,0,0,1) Details
15691747 Mashego MR, Jansen ML, Vinke JL, van Gulik WM, Heijnen JJ: Changes in the metabolome of Saccharomyces cerevisiae associated with evolution in aerobic glucose-limited chemostats. FEMS Yeast Res. 2005 Feb;5(4-5):419-30.


Exceptions to this were hexokinase, phosphofructokinase, pyruvate kinase and 6-phosphogluconate dehydrogenase of which the activities remained unchanged.
1(0,0,0,1) Details
15690349 Wittmann C, Hans M, van Winden WA, Ras C, Heijnen JJ: Dynamics of intracellular metabolites of glycolysis and TCA cycle during cell-cycle-related oscillation in Saccharomyces cerevisiae. Biotechnol Bioeng. 2005 Mar 30;89(7):839-47.


This agrees with previously observed metabolic control by phosphofructokinase and pyruvate kinase.
1(0,0,0,1) Details
17689798 Wittmann C, Weber J, Betiku E, Kromer J, Bohm D, Rinas U: Response of fluxome and metabolome to temperature-induced recombinant protein synthesis in Escherichia coli. J Biotechnol. 2007 Dec 1;132(4):375-84. Epub 2007 Jul 10.


The activation of the glycolytic flux was also indicated by the readjustment of glycolytic pool sizes leading to an increased driving force for the reaction catalyzed by phosphofructokinase.
1(0,0,0,1) Details