Protein Information

Name citrate lyase (protein family or complex)
Synonyms Citrate lyase; Citrate lyases

Compound Information

Name TCA
CAS 2,2,2-trichloroacetic acid

Reference List

PubMed Abstract RScore(About this table)
17227414 Hugler M, Huber H, Molyneaux SJ, Vetriani C, Sievert SM: Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in different lineages within the phylum Aquificae: evidence for two ways of citrate cleavage. Environ Microbiol. 2007 Jan;9(1):81-92.


Aquificaceae use citryl-CoA synthetase and citryl-CoA lyase, whereas Hydrogenothermaceae and 'Desulfurobacteriaceae' use ATP citrate lyase.
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18237306 Robidart JC, Bench SR, Feldman RA, Novoradovsky A, Podell SB, Gaasterland T, Allen EE, Felbeck H: Metabolic versatility of the Riftia pachyptila endosymbiont revealed through metagenomics. Environ Microbiol. 2008 Mar;10(3):727-37.


The population genome indicates that the symbionts use a partial Calvin-Benson Cycle for carbon fixation and the reverse TCA cycle (an alternative pathway for carbon fixation) that contains an unusual ATP citrate lyase.
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16501059 Srinivasan V, Morowitz HJ: Ancient genes in contemporary persistent microbial pathogens. Biol Bull. 2006 Feb;210(1):1-9.


Genomic annotations reveal the presence of the key enzymes of the rTCA cycle--citrate lyase (Enzyme Commission number EC 4.1.3.6) and 2-oxoglutarate synthase (EC 1.2.7.3)--along with the rest of the TCA cycle enzymes.
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16988757 Ferrera I, Longhorn S, Banta AB, Liu Y, Preston D, Reysenbach AL: Diversity of 16S rRNA gene, ITS region and aclB gene of the Aquificales. Extremophiles. 2007 Jan;11(1):57-64. Epub 2006 Sep 20.


In order to resolve the phylogenetic relationships within this group, three different phylogenetic datasets were used, namely the 16S rRNA gene, the intergenic transcribed spacer region between the 16S rRNA and 23S rRNA genes (ITS) and the gene coding for the ATP citrate lyase (aclB), a key enzyme in the reductive TCA cycle.
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18523725 Voordeckers JW, Do MH, Hugler M, Ko V, Sievert SM, Vetriani C: Culture dependent and independent analyses of 16S rRNA and ATP citrate lyase genes: a comparison of microbial communities from different black smoker chimneys on the Mid-Atlantic Ridge. Extremophiles. 2008 Sep;12(5):627-40. Epub 2008 Jun 5.

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