Protein Information

Name demethylase
Synonyms DMTase; Demethylase; MBD 2; MBD2; Methyl CpG binding domain protein 2; Methyl CpG binding protein MBD2; NY CO 41; Demethylases…

Compound Information

Name eugenol
CAS 2-methoxy-4-(2-propen-1-yl)phenol

Reference List

PubMed Abstract RScore(About this table)
10591845 Priefert H, Overhage J, Steinbuchel A: Identification and molecular characterization of the eugenol hydroxylase genes (ehyA/ehyB) of Pseudomonas sp. strain HR199. Arch Microbiol. 1999 Dec;172(6):354-63.

These genes were localized downstream of the eugenol catabolism genes vanA and vanB, encoding vanillate-O-demethylase, on an EcoRI fragment (E230) that has recently been cloned from a Pseudomonas sp. strain HR199 genomic library.
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8205227 Nagababu E, Lakshmaiah N: Inhibition of microsomal lipid peroxidation and monooxygenase activities by eugenol. Free Radic Res. 1994 Apr;20(4):253-66.

However, CumOOH supported monooxygenases (aminopyrine-N-demethylase and benzo (a) pyrene hydroxylase) required much higher concentrations of eugenol for inhibition.
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19835945 Ahmad A, Khan A, Manzoor N, Khan LA: Evolution of ergosterol biosynthesis inhibitors as fungicidal against Candida. Microb Pathog. 2010 Jan;48(1):35-41. Epub 2009 Oct 14.

Azoles target the ergosterol synthesizing enzyme lanosterol 14alpha-demethylase and are a widely applied class of antifungal agents.
Eugenol and methyl eugenol were reported to possess antimycotic properties.
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14511910 Shimoni E, Baasov T, Ravid U, Shoham Y: Biotransformations of propenylbenzenes by an Arthrobacter sp. and its t-anethole blocked mutants. J Biotechnol. 2003 Oct 9;105(1-2):61-70.

High conversion efficiencies were achieved in the biotransformations of isosafrole to piperonylic acid, and eugenol to a mixture of ferulic acid and vanillic acid.
The accumulation of the corresponding aromatic acids from the tested propenylbenzenes is due to the lack of m-demethylase activity in strain TA13 that prevents further cleavage of the benzene ring.
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8574354 Nagababu E, Sesikeran B, Lakshmaiah N: The protective effects of eugenol on carbon tetrachloride induced hepatotoxicity in rats. Free Radic Res. 1995 Dec;23(6):617-27.

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