Protein Information

Name P14
Synonyms HSPC003; p14; Late endosomal/lysosomal Mp1 interacting protein; MAPBPIP; Mitogen activated protein binding protein interacting protein

Compound Information

Name strychnine
CAS strychnidin-10-one

Reference List

PubMed Abstract RScore(About this table)
19332081 Mathers DA, McCarthy SM, Cooke JE, Ghavanini AA, Puil E: Effects of the beta-amino acid antagonist TAG on thalamocortical inhibition. Neuropharmacology. 2009 Jun;56(8):1097-105. Epub 2009 Mar 28.

We performed whole-cell recordings in 200-250 microm sections and immunocytochemical (ICC) studies in ventrobasal thalamus of rat brain (P12-P14).
Stimulation of medial lemniscus evoked inhibitory postsynaptic currents (IPSCs) which were purely glycinergic or GABA (A) ergic, or most commonly mixed glycinergic and GABA (A) ergic responses, based on abolition by strychnine, bicuculline, or combined antagonism.
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12888217 Kawa K: Glycine facilitates transmitter release at developing synapses: a patch clamp study from Purkinje neurons of the newborn rat. Brain Res Dev Brain Res. 2003 Aug 12;144(1):57-71.

Synaptic currents in immature Purkinje cells from rats on postnatal days 0-14 (P0-P14) were studied using whole-cell patch-electrodes applied to cerebellar slices (200 micro m in thickness).
Facilitatory effects of glycine were suppressed by strychnine (1 micro M), a specific blocker of the ionotropic glycine receptor, while the effects were also induced by other glycinergic agonists, including alpha-L-alanine (1 mM), L-serine (1 mM) and taurine (500 micro M).
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9614239 Kotak VC, Korada S, Schwartz IR, Sanes DH: A developmental shift from GABAergic to glycinergic transmission in the central auditory system. J Neurosci. 1998 Jun 15;18(12):4646-55.

Consistent with the electrophysiological observations, there was a reduction in staining for the beta2,3-GABAA receptor subunit from P4 to P14, whereas staining for the glycine receptor-associated protein gephyrin increased.
GABAergic and glycinergic components were identified by blocking transmission with bicuculline and strychnine (SN), respectively.
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16949227 Xu H, Wang W, Tang ZQ, Xu TL, Chen L: Taurine acts as a glycine receptor agonist in slices of rat inferior colliculus. Hear Res. 2006 Oct;220(1-2):95-105. Epub 2006 Sep 1.

We investigated in young rats (P10-P14) the effects of taurine on the neuronal responses and synaptic transmissions in the central nucleus of the inferior colliculus (ICC) with a brain slice preparation and with whole-cell patch-clamp recordings.
All these effects were reversible after washout and could be blocked by 3 microM strychnine, an antagonist of glycine receptors, but not by 10 microM bicuculline, an antagonist of GABA (A) receptors.
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14741396 Shibata S, Kakazu Y, Okabe A, Fukuda A, Nabekura J: Experience-dependent changes in intracellular Cl- regulation in developing auditory neurons. Neurosci Res. 2004 Feb;48(2):211-20.


Implanted strychnine pellets also prevented the decrease in [Cl-](i) in most neurons.
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