Protein Information

Name lysozyme
Synonyms LYZ; LZM; Lysozyme; Lysozyme C; Lysozyme C precursor; Lysozymes; Lysozyme Cs; Lysozyme C precursors

Compound Information

Name chloroform
CAS trichloromethane

Reference List

PubMed Abstract RScore(About this table)
18339394 Cao X, Hu G, Huo L, Zhu X, Li T, Powell J, Ito Y: Stationary phase retention and preliminary application of a spiral disk assembly designed for high-speed counter-current chromatography. J Chromatogr A. 2008 Apr 25;1188(2):164-70. Epub 2008 Mar 4.

The results indicated that the spiral disk assembly can produce excellent retention of stationary phase for moderately polar organic-aqueous solvent systems, such as chloroform-methanol-water (4:3:2) and hexane-ethyl acetate-methanol-water (1:1:1:1) by pumping lower mobile phase from head (H) to tail (T), and upper mobile phase from tail (T) to head (H) even at a high flow-rate (8 mL/min, Sf> 70%), regardless of whether the inlet is at the inner or outer terminal of the channel.
Acceptable resolutions were achieved when it was used for the separation of peptides such as Leu-Tyr and Val-Tyr, and proteins including cytochrome c and myoglobin, lysozyme and myoglobin, and fresh chicken egg-white proteins.
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20329549 Jin M, Zhao ZG, Qiu ZG, Wang JF, Chen ZL, Shen ZQ, Li C, Wang XW, Dong Y, Li JW: [Rapid method to extract high-quality RNA from activated sludge] . Huan Jing Ke Xue. 2010 Jan;31(1):260-5.

That is, the precipitated activated sludge was washed with TENP and PBS buffer, followed by using lysozyme and TRIzol to direct lysis of microbial cells, chloroform to remove protein and most of the DNA from bacterial lysate, isopropanol to precipitate nucleic acid and DNase I to hydrolyze residual DNA.
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19429273 Nyambura BK, Kellaway IW, Taylor KM: The processing of nanoparticles containing protein for suspension in hydrofluoroalkane propellants. Int J Pharm. 2009 May 8;372(1-2):140-6. Epub 2009 Jan 17.

Nanoparticles containing the model protein lysozyme have been produced using microemulsion and nanoprecipitation methods.
Freeze-drying water in oil emulsions, with chloroform as the organic solvent, followed by washing of excess surfactant (lecithin) led to the production of lactose nanoparticles having approximately 300 nm mean size.
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18489564 Schmidt RJ, Emara MG, Kung L Jr: The use of a quantitative real-time polymerase chain reaction assay for identification and enumeration of Lactobacillus buchneri in silage. J Appl Microbiol. 2008 Sep;105(3):920-9. Epub 2008 May 16.

METHODS AND RESULTS: DNA from silage samples spiked with different amounts of L. buchneri cells was isolated using a lysozyme/sodium dodecyl sulfate lysis and phenol/chloroform extraction method.
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17509707 Kaiser P, Surmann P, Vallentin G, Fuhrmann H: A small-scale method for quantitation of carotenoids in bacteria and yeasts. J Microbiol Methods. 2007 Jul;70(1):142-9. Epub 2007 Apr 21.

For disintegration and extraction, we combined primarily mild techniques: enzymatically we used combinations of lysozyme and lipase for bacteria as well as lyticase and lipase for yeasts.
For extraction we used a methanol-chloroform mixture stabilized efficiently with butylated hydroxytoluene and alpha-tocopherol.
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18572527 Shan G, Jin W, Lam EK, Xing X: Purification of total DNA extracted from activated sludge. J Environ Sci. 2008;20(1):80-7.

The effects of extraction buffers and lysis treatments (lysozyme, sodium dodecyl sulfate (SDS), sonication, mechanical mill and thermal shock) on yield and purity of the total DNA extracted from activated sludge were investigated.
Cetyltrimethyl ammonium bromide, potassium acetate and phenol/chloroform were used to remove proteins and polysaccharides from crude DNA.
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