Protein Information

Name RL1
Synonyms HP RNase; Ribonuclease; Ribonuclease A; Pancreatic ribonuclease; Pancreatic ribonuclease precursor; RIB 1; RIB1; RNASE1…

Compound Information

Name chloroform
CAS trichloromethane

Reference List

PubMed Abstract RScore(About this table)
17650497 Li WQ, Zhang GS, Wang K, Niu N, Pan DL: [An efficient method for isolation of mitochondrial DNA in wheat] . Yi Chuan. 2007 Jun;29(6):771-5.

The protocol consists of mitochondria isolation with differential centrifugation, Dnase I treatment, lysis with SDS and proteinase K, removing protein by TE-saturated phenol/chloroform extraction and a final RNase A treatment for obtaining mtDNA.
6(0,0,1,1) Details
19552787 Dussault D, Caillet S, Le Tien C, Lacroix M: Carotenoids' influence on radiotolerance of Pantoea agglomerans, a plant pathogen. Lett Appl Microbiol. 2008 Sep;47(3):208-13.

METHODS AND RESULTS: Pantoea agglomerans strains ATCC 49174 and RL1 were used for this study.
Total carotenoids were obtained by multiple extraction using chloroform/methanol (2 : 1), quantified by measuring the optical density at 453 nm and their antioxidant activity measured by a colorimetric method.
2(0,0,0,2) Details
19191968 Dussault D, Caillet S, Le Tien C, Lacroix M: Effect of gamma-irradiation on membrane fatty acids and peptidoglycan's muropeptides of Pantoea agglomerans, a plant pathogen. J Appl Microbiol. 2009 Mar;106(3):1033-40. Epub 2009 Jan 15.

METHODS AND RESULTS: Pantoea agglomerans strains ATCC 49174 and RL1 isolated from irradiated carrots were used for this study.
Total lipids were obtained by multiple extractions using methanol/chloroform (2 : 1) and were quantified by GC.
1(0,0,0,1) Details
17417019 Peirson SN, Butler JN: RNA extraction from mammalian tissues. Methods Mol Biol. 2007;362:315-27.

Purification of intact RNA is the primary step of many molecular biology techniques, including Northern blotting, RNase protection, quantitative polymerase chain reaction, and microarray assays.
RNA extraction is typically conducted using either a phenol-chloroform or a solid phase method.
1(0,0,0,1) Details